Biotin anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN95612

N° du produit (Fournisseur): 600-105-098

L’anticorps Chèvre Polyclonal anti-Biotin a été validé pour WB, ELISA et IHC. Il convient pour détecter Biotin dans des échantillons de .

Aperçu rapide pour Biotin anticorps (Alkaline Phosphatase (AP)) (ABIN95612)

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-Biotin anticorps (Alkaline Phosphatase (AP))

N° du produit (Fournisseur): 600-105-098

Fournisseur: Rockland

Fonction: Biotin Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.

Attributs du produit: Synonyms: goat anti-Biotin Antibody Alkaline Phosphatase Conjugation, Alk Phos conjugated goat anti-Biotin Antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Biotin Alkaline Phosphatase conjugated antibody has been tested by ELISA an is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:3,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Alkaline Phosphatase Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.